Catalogue# RPCA-Ubi: Rabbit Polyclonal Antibody to Ubiquitin-UBB/UBC
The Immunogen: Ubiquitin is a highly conserved globular 76 amino acid protein of about 8.5 kDa molecular weight. It has a important role in the targeting of proteins for proteolytic degradation. Proteins to be degraded are covalantly coupled to the C-terminus of ubiquitin by means of ubiquitin ligases. The ubiquitin itself is frequently also ubiquitinated, producing a polyubiquitin chain. The polyubquitinated complex is then recognized by a complex of degradative enzymes which together form the proteosome. Interestingly, ubiquitin also becomes covalently bonded to many types of pathological inclusions seen in serious human disease states which appear to be resistant to normal degradation, so that ubiquitin antibodies are very useful for studies of these inclusions. For example the neurofibrillary tangles and paired helical filaments diagnostic of Alzheimer's disease, the Lewy bodies seen in Parkinson's disease, and Pick bodies found in Pick's disease are all heavily ubiquitinated and can all be readily visualized with ubiquitin antibodies of appropriate specificity. Ubiquitin antibodies have become very widely used for such studies. The HGNC name for this protein is UBB, UBC.
Antibody Characteristics: Antibody was raised against glutaraldehyde cross linked ubiquitin in rabbit. Store at 4°C or -20°C. Avoid repeat freezing and thawing.
Suggestions for use: For immunofluorescence try RPCA-Ubi at dilutions of 1:500 to 1:1,000. For western blotting try RPCA-Ubi at 1:5,000 to 1:10,000.
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References: This antibody has not been formally characterized in a publication.
Limitations: This product is for research use only and is not approved for use in humans or in clinical diagnosis.
Availability and Price: Available for shipping now, $200 US per aliquot of 100 microliters of serum, enough for hundreds of experiments. For order form press here
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