Rabbit Polyclonal against Peripherin from EnCor Biotechnology Inc.


Catalogue RPCA-Peri: Polyclonal Antibody to Peripherin- PRPH The Immunogen: Peripherin is a ~57kDa intermediate filament subunit found initially in sensory neurons of the peripheral nervous systems, which gives the protein its name (1). The HGNC name for this protein is PRPH. Subsequently peripherin was found in some sensory and other neurons of the central nervous system and also in rat pheochromacytoma PC12 cells (2,3). Peripherin is also expressed in certain neuroendocrine tumors and in the insulin producing cells of the pancreas. Peripherin belongs to the Class III family of intermediate filament subunits which also includes vimentin, glial fibrillary acidic protein (GFAP) and desmin. Antibodies to peripherin can be used in identifying, classifying, and studying neurons throughout the nervous system. Peripherin is also a good diagnostic marker for ballooned axons seen in Lou Gehrig's disease (Amyotrophic lateral sclerosis) and some neuronally derived tumors. Autoantibodies to peripherin are frequently seen in the sera of patients with diabetes. Peripherin is not related to peripherin/RDS, a protein of the photoreceptor outer membrane mutations of which are causative of certain forms of slow retinal degeneration. The characterization of an antibody produced in the same way as RPCA-Peri has been published (4). The HGNC name for this protein is PRPH.

Left: Western blot of whole rat brain stem homogenate stained with RPCA-Peri, at dilution of 1:20,000. A prominent band running with an apparent SDS-PAGE molecular weight of ~57kDa corresponds to Peripherin. A lower band at ~48kDa is derived from the Peripherin molecule. Right: Mixed neuron/glia cultures from newborn rat brain stained with MCS-7C5 antibody to peripherin (green) and chicken polyclonal antibody to phosphorylated NF-H CPCA-NF-H (red channel). A class of large neurons, like the one in the middle of this image, contain peripherin, while the majority of neurons and their processes contain NF-L and not peripherin. Interestingly, the periperin positive cells often contain a cytoplasmic inclusion next to the nucleus which stains for both peripherin and NF-L, and so appears golden in this kind of image. The blue channel reveals the localization of DNA.
Antibody Characteristics: Antibody was raised in rabbit against recombinant full length peripherin purified from E. coli. The production and characterization of a similar but not identical antibody was described in reference 1 below. Store at 4°C or -20°C. Avoid repeat freezing and thawing. Suggestions for use: For immunocytochemistry on cells in tissue culture or in tissue sections, try this antibody at about between about 1:1,000 using fluorescent secondary antibodies or 1:5,000 using peroxidase or other enzyme linked methods. For immunoblotting 1:20,000 is recommended. Omim link: press here References: 1. Portier MM, de Néchaud B, Gros F. Peripherin, a new member of the intermediate filament protein family. Dev Neurosci. 6:335-44 (1984) 2. Troy CM, Brown K, Greene LA, Shelanski ML. Ontogeny of the neuronal intermediate filament protein, peripherin, in the mouse embryo. Neuroscience. 36:217-37 (1990). 3. Aletta JM, Angeletti R, Liem RK, Purcell C, Shelanski ML, Greene LA. Relationship between the nerve growth factor-regulated clone 73 gene product and the 58-kilodalton neuronal intermediate filament protein (peripherin).J Neurochem. 51:1317-20 (1988). 4. Errante LD, Wiche G and Shaw G. Distribution of plectin, an intermediate filament-associated protein, in the adult rat central nervous system J. Neurosci. Res. 37:515-528 (1994). Price and Availability: - We currently supply 100 microliter aliquots for $200. Material is in stock and ready for immediate shipping. Limitations: This product is for research use only and is not approved for use in humans or in clinical diagnosis. Use of Images or Text: The contents of this page are available for modification and reuse under the terms of the Creative Commons Attribution/Share-Alike License 3.0 and the GNU Free Documentation License, unversioned with no invariant sections, front-cover texts, or back-cover texts. These licences permit modification and reuse, even commercially, as long as authorship credit and a link to this page is given. ©EnCor Biotechnology Inc. .