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Protocols:

Formulations for commonly used buffers and media

Immunostaining of cells in tissue culture

Immunostaining of tissue sections

N-terminal Peptide Sequencing from SDS-PAGE Electroblots

SDS-Polyacrylamide Gel Electrophoresis

Using Microsoft Excel (tm) to interpret and plot ELISA data

Western blotting

 

Useful On-Line computer Programs:

Calculate how much Ammonium Sulfate to weigh out to make a solution of a specific level of saturation

Translate a protein sequence into a DNA sequence for optimal expression in different species

Calculate solution volumes to make SDS-PAGE resolving and stacking gels

Generate 60 base overlapping DNA oligonucleotides to synthesize longer sequences using the Gibson assembly

Calculate solution volumes to make SDS-PAGE resolving and stacking gels using Biorad 40% acrylamide

Calculate how much substance to weigh out to make a solution of a specific volume and molarity

Calculate the molecular weight of a mouse selected protein sequence

Calculate the absorbance coefficent of a protein at 280nm from the amino acid sequence

Go from protein sequence and OD280 determination to mg/ml protein concentration

Calculate the molecular weight of a mouse selected nucleic acid sequence

Centrifugation: To find the RCF given by a particular rotor at a particular RPM

Centrifugation: To find the RPM to set a particular rotor at for a specific RCF

Calculate the blood volume of a rodent from the body weight

 

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