The Immunogen: Pma1p is an abundant mutidomain protein of yeast which is localized in the plasma membrane. It functions as a major regulator of cytoplasmic pH, by pumping protons out of the cell. It is part of the P2 subgroup of cation-transporting ATPases. Since Pma1p is a major plasma membrane protein, antibodies which bind to it such as MCA-40B7 are useful markers of yeast plasma membranes.
Diagram of Domain Structure: Generated from sequence of yeast
Pma1p sequence CAA96708
(with the SMART program
from EMBL in Heidleberg).
From N-terminus (left in diagram) to C-terminus the domains are as follows.
Following two regions of low complexity (purple) is PFAM: Cation_ATPase_N
which refers to the Cation
ATPase N-terminal domain. PFAM:E1_E2 Atpase refers to the E1
E2 ATPase domain. PFAM Hydrolase refers to the Hydrolase
domain. Finally there are 6 predicted transmembrane segments (Blue).
Scale is number of amino acids;
The Antibody: Mouse monoclonal antibody, in the form of sterile-filtered cell culture fluid from an Integra CL-350 biochamber plus sodium azide.
Suggestions
for use: For western blots of yeast protein samples (cell lysates), try
MCA-40B7 diluted 1/10,000, followed by chemiluminescent detection (ECL). For
non-ECL western detection methods, try MCA-40B7 diluted 1/1,000 to 1/5,000.
For immunofluorescence on yeast cells, try MCA-40B7 diluted 1/500 to 1/5,000.
MCA-40B7 reacts with Pma1p based on the size of the band detected on western
blots and the immunofluorescence localization pattern. MCA-40B7 also detects
Pma1p-containing fusion proteins on western blots. Antibody preparation contains
10mM sodium azide preservative (Press here
for Material Safety Data Sheet). Avoid repeated freezing and thawing, store
at 4°C or -20°C.
References: The characterization of this antibody has not been formally published.
Limitations: This product is for research use only and is not approved for use in humans or in clinical diagnosis.
Availability and Price: Available for shipping now, $200 US per aliquot of 0.5 mls of material, enough for hundreds of experiments. For order form press here
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