Catalogue# MCA-3H11: Neurofilament NF-M Monoclonal Antibody Clone 3H11
The Immunogen:
Neurofilaments are the 10nm or intermediate filament proteins found specifically
in neurons, and are composed predominantly of three major proteins called NF-L,
NF-M and NF-H. NF-M is the neurofilament middle or medium molecular weight polypeptide
and runs on SDS-PAGE gels at 145-160 kDa, with some variability across species
boundaries. Antibodies to NF-M are useful for identifying neuronal cells and
their processes in tissue sections and in tissue culture. NF-M antibodies can
also be useful to visualize of neurofilament accumulations seen in many
neurological diseases, such as Amyotrophic Lateral Sclerosis (Lou Gehrig's disease) and Alzheimer's disease.
Left: Rat spinal cord homogenate showing the major intermediate filament proteins of the nervous system (lane 1). The remaining lanes show blots of this material stainted with various antibodies including MCA-3H11 (lane 3). Right: Culture of adult neural cells grown as described (2). Mature neurons can be identified by their morphology and because they stain strongly with antibodies to NF-L, NF-M and NF-H. The surrounding stellate red cells are stained with EnCor's rabbit polyclonal Rat facial nucleus before RPCA-a-Int. These are apparently mitotic neuronal progenitor cells and express many other neuronal markers.
Antibody characteristics: MCA-3H11 was raised against a recombinant fusion protein containing the extreme C-terminus of rat NF-M expressed in and purified from E. coli (1). The epitope is localized to within the last 56 amino acids at the extreme C-terminus of rat NF-M, the so-called KE segment which is highly conserved between NF-M molecules from different species. MCA-3H11 is an IgG1 class antibody with a k light chain. It recognizes intact NF-M strongly and specifically on immunoblots in all mammals and birds tested to date and also binds NF-M in cells in tissue culture and in sectioned material.
Suggestions for use: The antibody is supplied as 100 microliters of ascites fluid. The antibody works at a dilution of 1:500 for immunofluorescence microscopy and 1:5,000 for immunoblots.
Limitations: This product is for research use only and is not approved for use in humans or in clinical diagnosis.
OMIM link: press here
References:
1. Harris, J., Ayyub, C. and Shaw G. A molecular dissection of the carboxyterminal tails of the major neurofilament subunits NF-M and NF-H. J Neurosci Res 30:47-62 1991.
2. Evans J. et al. Characterization of mitotic neurons derived from adult rat hypothalamus and brain stem. J. Neurophysiol. 87:1076-85 (2002).
Availability and Price: Available for shipping now, $200 US per aliquot of 100 microliters of concentrated tissue culture material, enough for hundreds of experiments. For order form press here.
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