The Immunogen : This monoclonal was raised by injecting mice with yeast nuclear preparations and screening the resullting hybridomas by immunofluorescence on yeast cells. MCA-39C7 was one of a series of clones which strongly and specifically labelled the nuclear pore complex. When this antibody was tested on cells from other species, including rat, mouse and human cells, it has invariably strongly stained nuclear pore complexes, so it appears to be an excellent and panspecific marker for these important structures. MCA-39C7 does not work well on western blots so we are currently unsure of the exact identity of the protein to which it binds.

Antibody Characteristics: The antibody is provided as sterile-filtered cell culture fluid from an Integra CL-350 biochamber plus sodium azide. The IgG isotype is IgG1.

Suggestions for use: For immunofluorescence on yeast cells, try MCA-39C7 diluted 1:100 to 1:500. For immunofluorescence on mammalian cells try at 1:50 to 1:100. We estimate that the specific antibody concentration is about 0.25mgs/ml. Antibody preparation contains 10mM sodium azide preservative (Press here for Material Safety Data Sheet). Avoid repeated freezing and thawing, store at 4°C or -20°C.

References: The characterization of this antibody has not been formally published.

Limitations: This product is for research use only and is not approved for use in humans or in clinical diagnosis.

Availability and Price: Available for shipping now, $200 US per aliquot of 0.5ml of material, enough for hundreds of experiments. For order form press here