The Immunogen: Pdi1p is a yeast protein disulfide isomerase. It is a major endoplasmic reticulum protein with chaperone and co-chaperone activity, involved in retention of resident ER proteins. It also has a role in recognizing proteins targeted for ER-associated degradation (ERAD) and is a member of the protein disulfide isomerase family. Proteins in this family also can break disulfide bonds on substrate proteins. The protein is also known as Eps1p.

Antibody specificity (above right): The Western blots of whole yeast protein extracts with a collection of our antibodies. The blot for MCA-38H8 is in the indicated lane, and the number indicates the SDS-PAGE molecular weight in kiloDaltons.

<Antibody Characteristics: The antibody is at a concentration of 6.6 mg/ml in PBS + azide. It has been purified by protein G column chromatography and is substantially pure (>90%) based on SDS-PAGE analysis. The mAb has been stored at 4°C and has never been frozen. The antibody may be frozen at -70°C without loss of reactivity on a western blot. Western blots of yeast protein extracts probed with 38H8 diluted 1/20,000 (ECL detection) reveal a ~65 kDal band. Immunofluorescence using yeast cells with MCA-38H8 gives an endoplasmic reticulum staining pattern, and for this purpose should be used at a dilution of 1/500 to 1/5,000. The specificity of the mAb was determined by screening a yeast genomic library in phage lgt11. Four different clones from the library all contain different overlapping regions of Pdi1p. Pdi1p has a predicted protein size of 58.2 kDa. The subcellular localization of the the protein is the endoplasmic reticulum and nuclear envelope. Sterile-filtered cell culture fluid from an Integra CL-350 biochamber plus sodium azide. The isotype is IgG1-k.

Suggestions for use: Western blots of yeast protein extracts probed with MCA-38H8 diluted 1/20,000 (ECL detection) reveal a ~65 kDal band. Initial dilution range: 1/5,000 - 1/10,000. Press here for image of blot. Immunofluorescence using yeast cells with MCA-38H8 gives an ER staining pattern, and for this purpose should be used at a dilution of 1/500 to 1/5,000. Antibody preparation contains 10mM sodium azide preservative (Press here for Material Safety Data Sheet). Avoid repeated freezing and thawing, store at 4°C or -20°C.

Saccharomyces Genome Database (SGD) Link: Press here

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