Catalogue# MCA-34B12: Monoclonal Antibody 34B12 to Nop5p/Nop58p

The Immunogen: Nop5p (a.k.a. Nop58p), is localized in the nucleolus and required for pre-18S rRNA processing in Saccharomyces cerevisiae (baker's yeast).

We are OEM suppliers of this antibody- in this case researchers at the University of Florida generated this reagent, and we now manufacture it, have characterized it and generated the data presented on this page. This antibody is available from several other vendors, but we can supply it more cheaply and we can provide you with more detailed information on the properties of the antibody.

Antibody Specificity (left): Antibody recognizes a single 57kDa band in blots of whole yeast protein extracts.

The Antibody: Sterile-filtered cell culture fluid from an Integra CL-350 biochamber plus sodium azide. The antibody class is IgG1 and the antibody concentration is about 1mg/ml.

Suggestions for use: For western blots of yeast protein samples, use MCA-34B12 diluted 1/10,000, followed by chemiluminescent detection (ECL). Press here for image of blot. For immunofluorescence on yeast cells, use MCA-34B12 diluted 1/1,000 to 1/10,000. We routinely dilute to 1/2,000. For immunoprecipitations, try 2-4 µl for the amount of lysate derived from 5 OD600 units of yeast culture. MCA-34B12 does not immunoprecipitate as well as another Nop5p monoclonal antibody, MCA-37C12, which is also available from EnCor. Antibody preparation contains 10mM sodium azide preservative (Press here for Material Safety Data Sheet). Avoid repeated freezing and thawing, store at 4°C or -20°C.

UniProt/Swiss-Prot Link: press here

Reference: Wu P, Brockenbrough JS, Metcalfe AC, Chen S. and Aris JP. Nop5p is a small nucleolar ribonucleoprotein component required for pre-18 S rRNA processing in yeast. J. Biol. Chem. 273 (26), 16453-16463 (1998).

Limitations: This product is for research use only and is not approved for use in humans or in clinical diagnosis.

Availability and Price: Available for shipping now, $200 US per aliquot of 500ml of material, enough for hundreds of experiments. For order form press here

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