Catalogue# MCA-34B12: Monoclonal Antibody 34B12 to Nop5p/Nop58p
The Immunogen: Nop5p (a.k.a. Nop58p), is localized in the nucleolus and required for pre-18S rRNA processing in Saccharomyces cerevisiae (baker's yeast).
Antibody Specificity (left): Antibody recognizes a single 57kDa band in blots of whole yeast protein extracts.
The Antibody: Sterile-filtered cell culture fluid from an Integra CL-350 biochamber plus sodium azide. The immunoglobulin subtype and concentration of IgG are unknown.
Suggestions for use: For western blots of yeast protein samples, use MCA-34B12 diluted 1/10,000, followed by chemiluminescent detection (ECL). Press here for image of blot. For immunofluorescence on yeast cells, use MCA-34B12 diluted 1/1,000 to 1/10,000. We routinely dilute to 1/2,000. For immunoprecipitations, try 2-4 µl for the amount of lysate derived from 5 OD600 units of yeast culture. MCA-34B12 does not immunoprecipitate as well as another Nop5p monoclonal antibody, MCA-37C12, which is also available from EnCor. Antibody preparation contains 10mM sodium azide preservative (Press here for Material Safety Data Sheet). Avoid repeated freezing and thawing, store at 4°C or -20°C.
UniProt/Swiss-Prot Link: press here
Reference: Wu P, Brockenbrough JS, Metcalfe AC, Chen S. and Aris JP. Nop5p is a small nucleolar ribonucleoprotein component required for pre-18 S rRNA processing in yeast. J. Biol. Chem. 273 (26), 16453-16463 (1998).Limitations: This product is for research use only and is not approved for use in humans or in clinical diagnosis.
Availability and Price: Available for shipping now, $200 US per aliquot of 500ml of material, enough for hundreds of experiments. For order form press here
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