Catalogue# MCA-22G1: Monoclonal Antibody 22G1 to Nop2p
The Immunogen: Nop2p is the yeast homologue of the human p120 nucleolar "proliferation" antigen (1). The Nop2p protein is localized in the nucleolus and is involved in large ribosomal subunit assembly, and possesses a RNA methyltransferase activity (2,3).
Antibody Characterstics: Specificity was determined by western blot analysis of extracts from a yeast strain expressing Nop2p under GALUAS control, and from strains containing Nop2 ts alleles that express Nop2p isoforms of altered molecular weight. Sterile-filtered cell culture fluid from an Integra CL-350 biochamber plus sodium azide. The isotype is IgG1-K. The exact IgG concentration is not known.
Suggestions for use: For western blots of yeast protein samples, use 22G1 diluted 1/10,000 (cell lysates) to 1/25,000 (nuclear fractions), followed by chemiluminescent detection (ECL). For other (non-ECL) western detection methods, try 22G1 diluted 1/1,000 to 1/5,000. For immunofluorescence on yeast cells, use 22G1 diluted 1/500 to 1/5,000. We routinely dilute 22G1 to 1/1,000. Antibody preparation contains 10mM sodium azide preservative (Press here for Material Safety Data Sheet). Avoid repeated freezing and thawing, store at 4°C or -20°C.
Comprehensive Yeast Genome Database Link: press here
References:
1. de Beus E, Brockenbrough JS, Hong B & Aris JP. Yeast NOP2 encodes an essential nucleolar protein with homology to a human proliferation marker. J. Cell Biol. 127:1799-813 1994
2. King M, Ton D & Redman KL. A conserved motif in the yeast nucleolar protein Nop2p contains an essential cysteine residue. Biochem. J. 337:29-35 1999
3. Hong B, Wu K, Brockenbrough JS, Wu P & Aris JP. Temperature sensitive nop2 alleles defective in synthesis of 25S rRNA and large ribosomal subunits in Saccharomyces cerevisiae. Nucleic Acids Res. 29:2927-37 2001
Limitations: This product is for research use only and is not approved for use in humans or in clinical diagnosis.
Availability and Price: Available for shipping now, $200 US per aliquot of 0.5mls of concentrated tissue culture supernatant, enough for hundreds of experiments. For order form press here
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