Catalogue# MCA-1G1: Monoclonal Antibody clone 1G1 to yeast poly(A) tail binding protein Pab1p
The Immunogen: Pab1p is a protein which binds poly-A tails of yeast mRNA molecules, and is known as polyadenylate binding protein. It is also referred to as PAB1, ACBP, CST11 and MRNP and the systematic name is YER165w. It is they only yeast poly-A binding protein, and is found in both the cytoplasm and nucleus of yeast cells. The protein has a molecular weight of 64kDa, and runs on typical SDS-PAGE gels at 67kDa.
Antibody characteristics: MCA-1G1 is a IgG2a class antibody with a k light chain and was raised against a preparation of yeast proteins UV crosslinked to polyadenylated RNA. It reacts cleanly with ~67kDa band on immunoblots, a little higher than the genuine molecular weight which is 63kDa. The antibody diffusely stains both the nuclei and cytoplasm of wild type yeast cells (1).
Suggestions for use: Antibody is supplied as Integra CL-350 flask material, which is concentrated tissue culture supernatant. For immunofluorescence use MCA-1G1 diluted 1/500. For western blots try MCA-1G1 at dilutions of 1/1,000 to 1/5,000. Store at 4°C short term or -20°C long term. Antibody preparation contains 10mM sodium azide preservative (Press here to download a PDF of this information). Avoid repeated freezing and thawing.
Limitations: This product is for research use only and is not approved for use in humans or in clinical diagnosis.
References:
1. Hector RE, Nykamp KR, Dheur S, Anderson JT, Non PJ, Urbinati CR, Wilson SM, Minvielle-Sebastia L, Swanson MS. Dual requirement for yeast hnRNP Nab2p in mRNA poly(A) tail length control and nuclear export. . EMBO J. 21:1800-1810 (2002).
Comprehensive Yeast Genome Database Link: here
Saccharomyces Genome Database (SGD) Link: here
Availability and Price: Available for shipping now, $200 US per aliquot of 500 microliters of concentrated tissue culture derived material, enough for hundreds of experiments. For order form press here.
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