Catalogue# MCA-1E4: Monoclonal Antibody clone 1E4 to Yeast Nab1p/Npl3p/Nop3p, methylation specific
The Immunogen: Nab1p is one of the "heterogeneous nuclear RNA" or hnRNA binding proteins of yeast. Nab1p is also sometimes referred to as Npl3p, MTR13p, MTS1p and NOP3p and the systematic name is YDR432W. Nab1p is localized in the nucleus where it has a reticuled expression quite different from, for example Nop1p/fibrillarin.
Diagram of Domain Structure: RRM refers to an "RNA recognition motif", a ~90 amino acid motif found in many single strand RNA binding proteins. Lilac blocks are regions of low complexity, in this case rich in glutamine, proline or glycine. Scale is number of amino acids;
Antibody characteristics: MCA-1E4 is a IgG1 class antibody with a k light chain and was raised against a preparation of yeast proteins UV crosslinked to polyadenylated RNA. It reacts cleanly with ~50kDa band on immunoblots, a little higher than the genuine molecular weight which is 45kDa. The antibody stains the nuclei of yeast and mammalian cells in sections in tissue culture and on formalin fixed histological sections.
Suggestions for use: Antibody is supplied as Integra CL-350 flask material, which is concentrated tissue culture supernatant. For immunofluorescence use MCA-1E4 diluted 1/50 to 1/250. For immunocytochemistry with ABC or other enzymatic methods use MCA-1E4 at dilutions of 1:1,000. For western blots try MCA-1E4 at dilutions of 1/1,000 to 1/5,000. Store at 4°C short term or -20°C long term. Antibody preparation contains 10mM sodium azide preservative (Press here to download this information). Avoid repeated freezing and thawing.
Limitations: This product is for research use only and is not approved for use in humans or in clinical diagnosis.
Comprehensive Yeast Genome Database Link: Here
References:
1. Wilson SM, Datar KV, Paddy MR, Swedlow JR, Swanson MS. Characterization of nuclear polyadenylated RNA-binding proteins in Saccharomyces cerevisiae. J Cell Biol. 127:1173-84 (1994).
Comprehensive Yeast Genome Database (Munich): here
Yeast Genome database (Stanford) Link: here
Availability and Price: Available for shipping now, $200 US per aliquot of 500 microliters of concentrated tissue culture derived material, enough for hundreds of experiments. For order form press here.
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