Catalogue CPCA-MAP2: Chicken Polyclonal Antibody to Microtubule Associated Protein 2 (MAP2)
The Immunogen: Microtubules are 25nm diameter protein rods
found in most kinds of eukarytic cells. They are polymerized from a dimeric
subunit made of one a subunit and one b tubulin subunit. Microtubules are associated with a family of proteins called microtubule associated proteins (MAPs), which includes the protein t (tau) and a group of proteins referred to as MAP1, MAP2, MAP3, MAP4 and MAP5. MAP2 is made up of two ~280kDa apparent molecular weight bands referred to as MAP2a and MAP2b. A third lower molecular weight form, usually called MAP2c, corresponds to a pair of protein bands running at ~70kDa on SDS-PAGE gels. All these MAP2 forms are derived from a single gene by alternate transcription, and all share a C-terminal sequence which includes either three or four microtubule binding peptide sequences, which are very similar to those found in the related microtubule binding protein t (tau). MAP2 isoforms are expressed only in neuronal cells and specifically in the perikarya and dendrites of these cells. Antibodies to MAP2 are therefore excellent markers on neuronal cells, their perikarya and neuronal dendrites. In contrast t (tau) is found predominantly in neuronal axons
Left: Western blot of MAP2 in preparation. Right: View of mixed neuron/glial cultures stained with CPCA-MAP2 (red). The perikarya and dendrites of neurons are strongly and specifically stained with the MAP2 antibody, while the axons of the neurons and the processes of all other cell types in these cultures (astrocytes, oligodendrocytes, microglia, endothelia and fibroblasts) are all negative. Cell nuclei are visualized with DAPI DNA stain.
Antibody Characteristics: This antibody was generated in chicken by
standard procedures and immunoglobulin was extracted from egg yolk. The resulting
polyclonal antibody belongs to the IgY subclass. This is the chicken homologue
of mammalian IgG and can be used in the same general way, with the caveat that
this type of antibody does not bind either Protein A or Protein G. Suitable
second antibody reagents can be obtained from many vendors including Molecular
Probes and Sigma-Aldrich. Store at 4°C or -20°C. Avoid repeat freezing and thawing.
Suggestions for use: The IgY solution is at a concentration of ~19mg/ml and has an extremely high titre against MAP2. It can be used at dilutions of 1:5,000 and lower in immunofluorescence experiments.
Price and Availability: - We currently supply 50 microliter aliquots for $250. Material is in stock and ready for immediate shipping.
Limitations: This product is for research use only and is not approved for use in humans or in clinical diagnosis.
©EnCor Biotechnology Inc. .