Catalogue CPCA-CKIa: Chicken Polyclonal Antibody to Casein Kinase 1 alpha- CSNK1A1
The Immunogen: Many serine threonine (ser/thr) protein kinases are activated by specific small signalling molecules such as calcium, cAMP, cGMP while others appear to be cofactor independent. The casein kinases belong to this group, and how their activity is regulated is still something of a mystery (1,2). There are two broad families of these kinases called CKI and CKII (or CK1 and CK2). They were originally isolated since extracts of most cells contain two distinct fractions of kinase activaty both of which are able to phosphorylate the milk protein casein. It is now known that the CKI and CKII activities represent two different families of distinct kinases, each consisting of several different gene products. The different proteins clearly represent an ancient and unusually conserved family of kinases, sharing some unique sequence characteristics not found in other protein kinases. The CKI family consists of several different genes, divided in alpha, beta, gamma, delta and epsilon, some of which are encoded by more than one gene. The single mammalian CK1 alpha gene can generate four different proteins by alterate transcription. CPCA-CK1a was raised against the shortest of these proteins, and will therefore bind to all CK1 isotypes. The CK1 alpha proteins are involved in the control of protein degradation, and phosphorylate acidic regions of their substrate molecules. Typically the target ser/thr residues closely C-terminal to two or three aspartic or glutamic acid residues. Alternately, phosphorylation on residues 3 or 4 amino acids N-terminal to a ser/thr residue can also favor CKI phosphorylation. This means that CKI family enzymes often phosphorylate one or more ser/thr residues C-terminal to a phosphorylation site generated by the activity of another protein kinase. The HGNC name for this protein is CSNK1A1.
Left: Western blot of whole rat spinal cord homogenate stained with CPCA-CK1a, at dilution of 1:10,000. A prominent band running with an apparent SDS-PAGE molecular weight of ~38kDa and a less prominent band at ~34kDa corresponds to other CK1 alpha isotypes. Right: High power image of HeLa cells stained with chicken casein kinase 1 alpha antibody (red) and our panspecific mouse monoclonal antibody to nuclear pore complexes MCA-39C7 (green). Casein kinase 1 alpha has a particulate distribution both in the nucleus and the cytoplasm. Blue is the Hoeschst DNA stain. High quality immunocytochemical images made using this antibody are shown in reference 4.
Antibody Characteristics:This antibody was generated in chicken by standard procedures and immunoglobulin was extracted from egg yolk. The resulting polyclonal antibody belongs to the IgY subclass. This is the chicken homologue of mammalian IgG and can be used in the same general way, with the caveat that this type of antibody does not bind either Protein A or Protein G. Suitable second antibody reagents can be obtained from many vendors including Molecular Probes and Sigma-Aldrich. Store at 4°C or -20°C. Avoid repeat freezing and thawing.
Suggestions for use: Try at dilutions of 1:500 to 1:1,000 for immunofluorescence, and 1:5,000 for ABC or other enzyme linked immunocytochemical procedures. For western blots try at 1:10,000.
Omim link: press here
References:
1. Knippschild U, Gocht A, Wolff S, Huber N, Lohler J, Stoter M. The casein kinase 1 family: participation in multiple cellular processes in eukaryotes. Cell Signal. 17:675-89 (2005)
2. Vielhaber E and Virshup DM. Casein kinase I: from obscurity to center stage. IUBMB Life 51:73-8 (2001).
3. Kuret J, Johnson GS, Cha D, Christenson ER, DeMaggio AJ, Hoekstra MF. Casein kinase 1 is tightly associated with paired-helical filaments isolated from Alzheimer's disease brain. J Neurochem. 69:2506-15 (1997).
4. Dupre-Crochet S, Figueroa A, Hogan C, Ferber EC, Bialucha CU, Adams J, Richardson EC, and Fujita Y. Casein Kinase 1 Is a Novel Negative Regulator of E-Cadherin-Based Cell-Cell Contacts. Molecular and Cellular Biology Volume 27:3804-3816 (2007)
Price and Availability: - We currently supply 100 microliter aliquots for $200. Material is in stock and ready for immediate shipping.
Limitations: This product is for research use only and is not approved for use in humans or in clinical diagnosis.
Use of Images or Text: The contents of this page are available for modification and reuse under the terms of the Creative Commons Attribution/Share-Alike License 3.0 and the GNU Free Documentation License, unversioned with no invariant sections, front-cover texts, or back-cover texts. These licences permit modification and reuse, even commercially, as long as authorship credit and a link to this page is given.
©EnCor Biotechnology Inc. .