Crude homogenates of rat facial nucleus were run out on 7.5% SDS-PAGE gels and electrophoretically transferred to nitrocellulose. The nitrocellulose membranes were then blotted with MCA-2E3 monoclonal antibody to a-internexin, and binding of antibody to the appropriate band was visualized using chemiluminescence reagents. Protein in 10 lanes correspond to pairs of samples taken at 3, 5, 7, 14 and 28 days after experimental injury. C and E refer to control and experiment. Note the strong and clean single band running at ~66kDa, and the complete absence of background or nonspecific staining, despite the fact that the a-internexin protein is a relatively minor component of the crude homogenate. Link here back to the EnCor Biotechnology Home Page.
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